In vitro Micropropagation of Berberis chitria (Lindl.) - A Rare Medicinal Plant from Himachal Pradesh, India

نویسندگان

چکیده

An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and nodal explants. The effects of different concentrations BAP GA3 seed germination was investigated consequently 6.0 mg/l + 0.0 reported to be excellent germination. Callus induction leaf explants aseptically grown seedling were also studied by various 2, 4-D showing 94.26 ± 0.12% response at the concentration 2.0 0.4 4-D. highest percentage multiple shoot obtained (100.00 0.01% with an average 4.50 0.3 shoots) on MS medium augmented 1.0 Kn IAA. Whereas, combined NAA IBA 0.5 IAA showed (76.05 0.09%) root 3.84 0.27 roots per shoot. survival rate these plantlets under greenhouse condition 85%. This research communication provides first record about multiplication B. chitria, collected Pradesh Himalayas India. Plant Tissue Cult. & Biotech. 32(2): 227-236, 2022 (December)

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ژورنال

عنوان ژورنال: Plant Tissue Culture and Biotechnology

سال: 2022

ISSN: ['1818-8745', '1817-3721']

DOI: https://doi.org/10.3329/ptcb.v32i2.63556